Digital holographic microscopy dhm is a technique that uses optical interference patterns to record a threedimensional optical field for imaging, sensing, and microscopy applications. Lensless microscopy of cell cultures 2014 wiley analytical science. Continuous livecell culture imaging and singlecell tracking by computational lensfree led microscopy, sensors 2019. Osa color lensless inline holographic microscope with. In conclusion, we reported a lensless holographic superresolution microscope which is quite robust, easy to use, portable 95 g and costeffective, achieving a resolution of software platform converts existing 2d medical imaging data such as mri and. Tiny lensless endoscope captures 3d images of objects. Digital inline holography dihm is an imaging modality within the fast. Spotlight including stock price, stock chart, company news, key statistics, fundamentals and company profile. Software algorithms are written to automatically identify the nematode eggs from low resolution holographic videos or images captured from a scanner. Dlhm is a lensless methodology of imaging in two stages. Judy yee, professor and vice chair of radiology and biomedical imaging and chief of radiology at sfvamc, described echopixels true 3d software.
Basic lensless imaging for lowcost microscopy hackaday. Computational microscopy, sensing and diagnostics for telemedicine and global health applications. Presenter pierrealexandre blanche will discuss the application of holography in all of these areas and the technological advantages of holography over other techniques. Interfacing the device with koala software from lynceetec is considered in. The syringe pump was turned on at a flow rate of 1 ml per hour. The innovative new technology takes data from ct and mri scans and transforms it into 3d holographic images using 3d glasses, a laser stylus, and a special display. We experimentally built and tested a lensless imaging system where a dnn was trained to recover phase objects. Introduction digital inline holography dihm is an imaging modality within the fast evolving. Pedrini, w osten 2005 application of shortcoherence digital holography in microscopy. Spotlight stock quote phase holographic imaging phi.
Tiziani 2002 shortcoherence digital microcopy by use of lensless holographic imaging system, appl. Micromanager is an open source platform for controlling microscope hardware, automating image acquisition, and generating metadata about how images are acquired. Portable lensless widefield microscopy imaging platform. In the literature, there is a significant number of approaches developed upon the traditional holography principle, where a splitter is commonly used to separate object and reference beams 41. Biomedical optical phase microscopy and nanoscopy, elsevier published in 20 isbn. Improving lensless computational microscopy technology. Digital lensless holographic microscopy and applications. Here, we demonstrate for the first time to our knowledge that deep neural networks dnns can be trained to solve endtoend inverse problems in computational imaging. A lensless microscope capable of generating holograms of biotargets with. A lensless inline digital holographic microscopy system is proposed and its performance for dynamic 3d imaging and micromeasurements is. Depth resolved imaging using multiangle lensless holography.
The aim of the present work is to develop digital dynamic holography as a metrological tool for both transmission and reflection applications. Osa multiangle lensless digital holography for depth. The cooling of the cmos camera defines the local temperature of the integrated cell culture chamber. The principles and some applications of the digital lensless holographic. Fast 3d imaging with lensless holographic endoscopy employing coherent fiber bundles, by juergen w. Our lensless system consists solely of a diffuser placed in front of a standard image sensor. Holographic pixel superresolution in portable lensless on. Pdf lensless multispectral digital inline holographic microscope. In this paper, an irregular displacementbased lensless widefield microscopy imaging platform is presented by combining digital inline holography and. Science foundation nsf cbet division biophotonics program, the nsf emerging frontiers in. Using holography instead of optical focusing enables to build a costefficient, robust and compact microscope. Lensless inline dhm is the simplest arrangement, requiring no lenses, no mirrors, and typically only a light source, sample, and a digital imager chip.
Holopy digital holography and light scattering tools in python. Compact lensless digital holography infoscience epfl. But, if the object of study is transparent like, for instance, many living cells, photographic images cant be captured unless one uses special coloring agents. By combining digital holography with optical coherence tomography oct in a process known as holoscopy, 3d parallel image acquisition with extremely high data throughput at high imaging speeds is possible due to the lack of moving elements. For weaklyscattered amplitude object, we show that the. Project basic lensless imaging for lowcost microscopy. The software also supports 3d imaging from the reconstructed stack of. After the capture of the multiangle lensfree holographic images of the sample volume as illustrated in fig. A 3d printable platform for lensless holographic cellular imaging with open accessible software solutions has been developed delivering spatial resolutions of 1.
Portable lensless widefield microscopy imaging platform based on. After traveling a distance r, the phase of the reference beam in the plane of the. A multiangle lensfree holographic imaging platform that can accurately characterize both the axial and lateral positions of cells located within multilayered microchannels is introduced. A 3d printable platform for lensless holographic cellular imaging with open.
The details of the depth resolved imaging process using multiangle lensfree holography is summarized in fig. An introduction to lensless digital holographic microscopy. Lensless inline digital holography for dynamic applications. A multimodal novel lensless microscopy technology for. In this paper, an irregular displacementbased lensless widefield microscopy imaging platform is presented by combining digital inline holography and computational pixel superresolution using. Ozcan, partiallycoherent lensless holographic microscopy for automated semen analysis on a chip. After sample cleaning using 50% optiprep by volume, a 1 ml solution of the interface layer was loaded into a syringe. In this platform, lensfree digital holograms of the microobjects on the chip are recorded at different illumination angles using partially coherent illumination. Holoscopy makes ultrafast lensless imaging of scattering tissues possible.
Lensless microscopes, just like holographic ones, have several advantages. Lensless singleexposure 3d imaging nick antipa, grace kuo, reinhard heckel, ben mildenhall, emrah bostan, ren ng, and laura waller. Czarske, elias scharf, and robert kuschmierz, will be presented monday, 16. Holograms can also be used for integral imagingbased 3d display, lensless projection, and lidar beam steering. The principle of holographic imaging invented many decades ago has significantly advanced since the original digital inline holographic experiment 2,40. The presented 3dprintable portable opensource platform represents a fullyopen design, lowcost modular and versatile imagingsolution for use in high and lowresourceareasoftheworld. We propose a method for acquiring color images with a lensless inline holographic microscope lihm using sunlight illumination, which is suitable for weakly scattered amplitude objects. Digital holography systems that employ shadow imaging projection of 2d. Partially coherent lensless holographic microscopy with.
This incoherent holographic imaging and diagnostic modality has orders of. The dispensing needle of the syringe was connected to the input port of the microfluidic flow chip. Deep learning has been proven to yield reliably generalizable solutions to numerous classification and decision tasks. In biomedical imaging research, it serves as an incredibly important tool because it is free and open source, which means that scientists can benefit from the contributions of others to the software without paying costly licensing fees. The ozcan research group refereed journal publications. The lensless cellmicroscope combines holographic imaging of cells with. Automated image analysis systems to quantify physical and. Osa lensless computational imaging through deep learning. A new lensless imaging system is built to record holographic videos of soil particles flowing through microscale flow assays.
154 615 7 479 1500 1033 768 1131 445 95 1062 1052 1438 1413 619 734 1 1342 444 759 988 1288 612 467 1041 1067 423 1195 1237 1365 955 1377 1050 306 773 1296 1156 1042 488 1416 722